Fig 1: Confirmation of the iTRAQ-MS data by western blotting or real-time RT-PCR. (A) Western blot analysis of NF-?B1, RelA, MHC-I, RPS29, and NF?BIB in PS-infected and control samples at 12 and 24 hpi. GAPDH was served as internal reference. (B) The intensity ratio of the corresponding bands (infection/mock) was quantified using ImageJ software and normalized against GAPDH. (C) Eight selected differently expression proteins related to NF-?B pathway were testified using real-time RT-PCR method. Each gene was performed in three independent experiments. The relative gene expression was calculated using 2-??CT model, representative of n-fold changes in comparison with mock-infected samples. Error bars represent the standard error for triplicate samples. *P < 0.05; **P < 0.01; ***P < 0.001. The data was analyzed by two-way ANOVA followed by Duncan's test.